ManuScript Details
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Paper Id:
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IJCIRAS1067
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Title:
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INSILICO STUDY OF MIR-132 WITH ACETYLCHOLINESTERASE TO INVESTIGATE THE BINDING AFFINITY FOR INTERACTION
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| Published in: |
International Journal Of Creative and Innovative Research In All Studies |
| Publisher: |
IJCIRAS |
| ISSN: |
2581-5334 |
| Volume / Issue: |
Volume 1 Issue 7 |
| Pages: |
9
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| Published On: |
2/4/2019 8:38:23 AM (MM/dd/yyyy) |
| PDF Url: |
http://www.ijciras.com/PublishedPaper/IJCIRAS1067.pdf |
Main Author Details
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Name:
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Sabahat Sohail |
| Institute: |
PMAS-AAUR RAWALPINDI |
Co - Author Details
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Author Institute |
Abstract
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Research Area:
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Computational & Systems Biology |
| KeyWord: |
Acetylcholinesterase, Active site gorge, Huperzine A, miRNA-132, Comparative Modeling, Binding Affinity |
| Abstract: |
Acetylcholinesterase (AChE) is a key enzyme in central nervous system which hydrolaze neurotransmitter acetylcholine (ACh) at cholinergic brain synapses. High expression of AChE in brain and muscles is responsible for diverse neurological conditions leading from neuromuscular, neurodegenerative and neuropsychological diseases. miRNA-132 is a non-coding RNA molecule which help to regulate the expression level of number of genes involved in neurological development, angiogenesis and synaptic transmission. However, miR-132 is the only miR so far that has been experimentally validated as targeting AChE, with consequences on inflammatory responses. Various bioinformatics tools like MODELLER, I-Tasser, ModeRNA, 3D-Dart, ERRAT, ProSA and Rampage were employed for AChE and miR-132 model building and their validations. The binding affinities of these macromolecules were also investigated. The results revealed that the miR-132 binds to 3’-UTR region of AChE. The catalytic triad Ser-203, His-447 and Glu-334 found in active site gorge of protein binds to the ligand. Thus the models of protein-ligand and AChE-miR-132 showed a potential in interaction. Therefore miR-132 can be a potent inhibitor for Acetylcholinesterase and further analysis of this inhibitor could be helpful for exploration of ligand binding pockets and designing of a novel therapeutic target to cure central nervous system disorders. |
Citations
Copy and paste a formatted citation or use one of the links to import into a bibliography manager and reference.
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IEEE
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Sabahat Sohail, "INSILICO STUDY OF MIR-132 WITH ACETYLCHOLINESTERASE TO INVESTIGATE THE BINDING AFFINITY FOR INTERACTION", International Journal Of Creative and Innovative Research In All Studies,
vol. 1, no. 7, pp. 30-38, 2018.
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MLA
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Sabahat Sohail "INSILICO STUDY OF MIR-132 WITH ACETYLCHOLINESTERASE TO INVESTIGATE THE BINDING AFFINITY FOR INTERACTION." International Journal Of Creative and Innovative Research In All Studies,
vol 1, no. 7, 2018, pp. 30-38.
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APA
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Sabahat Sohail (2018). INSILICO STUDY OF MIR-132 WITH ACETYLCHOLINESTERASE TO INVESTIGATE THE BINDING AFFINITY FOR INTERACTION. International Journal Of Creative and Innovative Research In All Studies,
1(7), 30-38.
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INSILICO STUDY OF MIR-132 WITH ACETYLCHOLINESTERASE TO INVESTIGATE THE BINDING AFFINITY FOR INTERACTION
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